Active Interleukin 4 (IL4)
BSF1; BCGF1 ;B Cell Stimulatory Factor 1; Lymphocyte stimulatory factor 1; B Cell Growth Factor; Binetrakin; Pitrakinra
- Product No.APA077Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point9.3
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 208 For more details, please contact local distributors!US$ 520 For more details, please contact local distributors!US$ 1040 For more details, please contact local distributors!US$ 3120 For more details, please contact local distributors!US$ 7800 For more details, please contact local distributors!
ACTIVITY TEST of the Active Interleukin 4 (IL4)
Interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cellsand monocyte. As reported, IL-4 would induce the differentiation of THP-1 cells into dendritic cells and macrophages in vitro. In house data was obtained by the following experiment :THP-1 cells were cultured in RPMI-1640 and stimulated with 2ng/mL IL-4, after 7 days of stimulation, cell bodies enlarged with extending pseudopodia, and vesicular bodies appeared within the cells, which showed a morphological characteristics of dendritic cells and macrophages.<br/>Cell differentiation of THP1 cells after incubation with IL4 (2ng/mL) for 7 days was shown in Figure 1.
USAGE of the Active Interleukin 4 (IL4)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Interleukin 4 (IL4)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Interleukin 4 (IL4)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
BCA Protein Quantification KitMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
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