Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor
- Product No.APA045Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.2
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM10µg50µg200µg1mg5mg
- FOBUS$ 216 For more details, please contact local distributors!US$ 540 For more details, please contact local distributors!US$ 1080 For more details, please contact local distributors!US$ 3240 For more details, please contact local distributors!US$ 8100 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- SDS-PAGE
- Figure. Western Blot; Sample: APA045Hu61; Antibody: PAA045Hu06
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
TF-1, the human erythroleukemia cell line,provides a good system for detecting the activity of GM-CSF for it is a cell line of immature erythroid origin that completely depends on interleukin 3 (IL-3) or granulocyte-macrophage colony-stimulating factor (GM-CSF) for long term growth. As reported, GM-CSF was also able to induce differentiation of human monoblastic leukemia cell line U937. In house data was obtained by the following experiment: TF-1 cells and U937 cells were incubated in the presence of various concentrations of rhGM-CSF, then cells were observed by inverted microscope everyday . Cell proliferation of TF1 cells after incubation with GM-CSF (10ng/mL) for 3 days was shown in Figure 1.
Cell differentiation of U937 cells after incubation with GM-CSF (10ng/mL) for 5 days was shown in Figure 2.
USAGE of the Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
Polyacrylamide Gel Electrophoresis (PAGE) Experiment Service
INCREMENT SERVICES
BCA Protein Quantification KitMolecular Mass Marker for ProteinMonoclonal Antibody Customized ServicePolyclonal Antibody Customized ServiceProtein Activity Test Experiment ServiceElectrophoretic Mobility Shift Assay (EMSA) Experiment ServiceBufferLentivirus Packaging Experiment ServiceAdenovirus Packaging Experiment ServiceReal Time PCR Experimental ServiceSpike RBD Protein (S-RBD)Protein GProtein A
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